TECHNICAL DISCUSSION
RSI, Volume, Doses of Platelets, Concentration and Purity
“We know nothing, we've got everything to learn.”
Volume of Sampling: Quantity of Platelets
The term platelet rich plasma supposes an enrichment in platelets, above the usual concentration of 145 to 400 10^09/l.
The dose of platelets, and therefore the amount of released growth factors, depends directly on the volume of the sample, the platelet recovery rate, and the volume of PRP obtained.
The PRP applications are so broad that it is difficult to speak of an ideal volume. The minimum volume can, however, be defined in terms of the minimum requirement for platelet doses around 1 to 2 billion (1,2). Since the general PRP equation (3) tells us that the platelet dose is related to the RSI of a device and the volume of the PRP produced, it can be estimated that a useful device has an RSI greater than or equal to 3 or 4. Please refer to our general comparison (4) to see the current market offer summarized in a single table.
Most manufacturers only emphasize the concentration factor, to the detriment of platelet dose, volume and purity of PRP. We will discuss now once and for all the concept of concentration, which is a poor indicator of PRP.
1. https://pubmed.ncbi.nlm.nih.gov/29366744
2. https://pubmed.ncbi.nlm.nih.gov/31577658
Old gel tubes generating poor PRP
Platelet Dose
The WorldPRP kit represents a good compromise with 25 ml of volume, which makes it possible to obtain useful doses of 2.75 to 9 billion platelets. No other device can match this data.
Of course it's possible, and even advised in some cases as for the treatment of the scalp, to use two kits.
In that case, the platelet recovery rate would be about 87%.
See the comparisons to discuss these concepts in detail.
Purity: RBC
We can find very expensive devices on the market capable of automatically concentrating the platelet rate up to 5x, giving a platelet dose of about 5 billions, but they all have one thing in common: the PRP is systematically contaminated (1) by red blood cells. This should be avoided as much as possible in order to prevent ferrous deposits and other inflammatory disorders. Given the price of this equipment and their consumables, it is probably not a good deal.
Some devices are able to deliver a pure PRP - low rate of RBC. This is the case of the gel tubes. However a good concentration rate or a sufficient platelet dose cannot be obtained. Moreover, gel particles of acrylate are undoubtedly injected to the patients. What about the safety of this substance?
The WorldPRP kit is a tailor-made solution giving you the power to define the dose and the concentration without any red blood cell contamination. The purity of the PRP is more than 95%.
Quality: Leukocytes free
There is currently a debate about whether leukocytes are to be preserved or not, mainly in the orthopaedics. (1)
The PRP harvested with WorldPRP tubes is leukocytes free.
